WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation) Service
WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation) Service
WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation) Service
Whole-genome bisulfite sequencing (WGBS) and Enzymatic Methylation (EM-seq) are the methods of choice for obtaining a comprehensive DNA methylation profiling, evaluating the methylation patterns of nearly every CpG site of the entire genome. By comparing the proportion of unconverted and converted cytosines at the same location, the methylation levels are determined with accuracy. Genome-wide methylation sequencing (WGBS and EM-seq) provides deep insights into gene regulation, allowing identification of novel epigenetic markers and targets for disease.
In-depth DNA methylation analysis
- Very powerful solution for genome-wide biomarker discovery compatible with low input and highly fragmented cfDNA and FFPE samples
- Single-nucleotide resolution
- Evaluation of the methylation status of nearly every CpG sites of the entire genome
- Detection of global methylation patterns including outside of CpG islands and in low-CpG-density regions
- Identification of regions or even loci with differential methylation between groups using bioinformatics tools
- Identification of methylation pattern/signature predictive and discriminating different groups with Data Mining approach
- Providing a better understanding of development and disease
Comprehensive end-to-end service
- From DNA QC to sequencing raw data
- A dedicated scientist driving your project with high touch communication
- High-quality bioinformatic support (on request) to assist you with data analysis
WORKFLOW:
QC of the genomic DNA
- Measurement of DNA concentration
- Assessment of DNA quality
Library Preparation:
WGBS
- gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)
- Bisulfite conversion
- Library preparation
- QC of the WGBS libraries (DNA concentration, analysis of the profile)
EM-seq
- gDNA shearing on Bioruptor Pico (not necessary for cfDNA or FFPE)
- Library preparation with Enzymatic conversion
- QC of EM-seq libraries (DNA concentration, analysis of the profile)
Deep sequencing
- Samples are sequenced on Illumina platform, paired-end reads of 150bp length (PE150)
- 400M raw reads on average per samples (when pooling 6 samples/lane)
- Theoretical Coverage >30X for human, mouse and rat samples
- Detection of >50 million CpGs with 6-9X average CpG coverage for human samples
Bioinfomatic analysis
Analysis |
Features |
| Standard | Standard files provided:
|
| Differential methylation analysis | Identification of differentially methylated CpGs between sample groups.
Files provided:
|
| Gene ontology terms analysis | Enrichment analysis on gene sets. Gene Ontology terms that are overrepresented in differentially bound regions may indicate the underlying biological processes involved. |
| Pathway analysis | Identify biochemical pathways in which genes associated with differentially methylated regions (or individual differentially methylated CpGs) may be overrepresented. |
| G02040000 | WGBS (Whole Genome Bisulfite Sequencing) and EM-seq (Enzymatic Methylation) Service | Custom |
Related Products
Data sheet
Video
Contact form
