Kits estrazione per KingFisher Flex

• Specifiche: Automated high-throughput application: Purification of up to 96 samples using the KingFisher FLEX (Thermo Fisher Scientific)
  Ideally suited for veterinary diagnostics
• Materiale di partenza:
  Cell-free body fluids (200 μl)
  Whole blood (200 μl)
  Fecal samples (50-100 mg)
  Swabs from nasopharyngeal samples (e.g. Influenza testing)
  Tissue samples (up to 10 mg)
• Tempi di estrazione: 60 minuti
• Resa media: in base alla tipologia e quantità materiale di partenza
• Volume eluizione: 1.8 – 2.0

• Specifiche: Use of the KingFisher FLEX automation system for running up to 96 samples in parallel
  Automated elution into a 96-well plate
  Includes Carrier Mix with internal DNA and RNA extraction control
• Materiale di partenza:
  Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
  Cell culture supernatant, enrichment medium (up to 200 µl)
  Tissues (approx. 1 – 5 mg)
  Swab samples
  Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Manual lysis: tissue samples only (30 – 60 minutes)
  KingFisher FLEX lysis protocol: approx. 20 minutes
  KingFisher FLEX protocol: approx. 37 min.

CE-IVD

• Specifiche: Use of the KingFisher FLEX automation system for running up to 96 samples in parallel
  Automated elution into a 96-well plate
  Includes Carrier Mix with internal DNA and RNA extraction control
• Materiale di partenza:
  Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
  Cell culture supernatant, enrichment medium (up to 200 µl)
  Tissues (approx. 1 – 5 mg)
  Swab samples
  Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Manual lysis: tissue samples only (30 – 60 minutes)
  KingFisher FLEX lysis protocol: approx. 20 minutes
  KingFisher FLEX protocol: approx. 37 minutes

• Materiale di partenza:
  Sangue fresco o congelato fino a 400 μL Stabilizzato in EDTA o citrato
• Tempi di estrazione: KingFisher FLEX lysis protocol: approx. 25 minutes
  KingFisher FLEX isolation protocol: approx. 37 minutes
• Resa media: in base alla tipologia e quantità materiale di partenza: Sangue intero fino a 10 μg
  Purezza media: 1.7 – 2.0

• Specifiche: For use with the KingFisher ml machines
  Processing of up to 15 samples in parallel
• Materiale di partenza:
  Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
  Cell culture supernatant, enrichment medium (up to 200 µl)
  Tissues (approx. 1- 5 mg)
  Swab samples
  Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Lysis: approx. 15 – 30 minutes, up to 60 minutes for tissue samples
  KingFisher ml protocol: approx. 30 minutes

• Specifiche: Up to 15 samples can be processed in parallel
  Optimized for KingFisher ml automation systems
  Includes CarrierMix with internal DNA and RNA extraction control
• Materiale di partenza:
  ƒCell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl)
  Cell culture supernatant, enrichment medium (up to 200 µl)
  Tissue samples (approx. 1- 5 mg)
  Swab samples
• Tempi di estrazione: Lysis: approx. 15-30 minutes, up to 60 minutes for tissue samples. KingFisher ml protocol: approx. 30 minutesQuality of viral nucleic acids
  Tested with positive results in cDNA synthesis, PCR and TaqMan real-time PC