Bioruptor ® Plus
Bioruptor ® utilizza un unico sistema per processare in modo uniforme campioni multipli in tubi chiusi con capacità da 0.5 ml a 50 ml. Il sistema di raffreddamento integrato (water cooler e Single Cycle Valve) assicura elevata precisione di controllo della temperatura con conseguente precisione di qualità dei campioni. Uno strumento eccellente per lo shearing della cromatina, per la distruzione di cellule e tessuti e per molte altre applicazioni.
- Controllo Temperatura
- Di facile utilizzo
- Processa da 3 a 12 campioni
- Volume campioni da 100μl a 20ml
Applicazioni
- Shearing Cromatina
- ChIP-qPCR
- Estrazione RNA
- Estrazione Proteine
- Estrazione FFPE DNA
Testimonial
To expedite the larger-scale phenotyping of pollen number difference within Arabidopsis family, we have modified an existing method more suitable for our purpose of pollen counting using a Bioruptor®. We grew four plants per genotype. Three flower buds per plant were harvested and dried at 65°C overnight. We collected flower buds with mature pollen, but before the anthers were opened (flower stage 13). We did not use the first and second flowers of the inflorescence, as they tend to show developmentally immature flower morphologies. 30 μl of 5% Tween-20 is added to the dried flower and samples are sonicated using the Bioruptor® Plus with high power mode for 10 cycles (sonication cycle: 30 sec ON, 30 sec OFF) to separate pollen grains from the anthers. Then, all pollen solutions are measured with a cell counter.
Misako Yamazaki, Evolutionary and Ecological Genomics (Kentaro Shimizu group), Institute of Evolutionary Biology and Environmental Studies, University of Zurich, Switzerland
| # | Codice Prodotto | Descrizione | Formato |
|---|---|---|---|
| 1 | B01020001 | Bioruptor Plus® device with 1.5 ml (6 samples) tube holder & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 100 µl – 300 µl | 1 unità |
| 2 | B01020002 | Bioruptor Plus® device with 1.5 ml (6 samples) and 15 ml tube holders & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 100 µl – 2 ml | 1 unità |
| 3 | B01020003 | Bioruptor Plus® device with 0.5 ml (6 samples) tube holder & temperature controlled system (water cooler + single cycle valve) for recommended sample volume 50 µl – 100 µl | 1 unità |
Prodotti Correlati
 C20000020 Protein Extraction Kit for Bioruptor Plus |
 C20000010 RNA Extraction kit for Bioruptor Plus |
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