DNA and RNA purification
Diagenode offers a variety of optimized DNA purification methods allowing for recovery of high quality DNA after chromatin immunoprecipitation or methylated DNA immunoprecipitation, essential for ideal results in downstream applications such as qPCR or next generation sequencing. Our purification technology in our signature IPure kit utilizes a magnetic bead affinity method, enabling low input recovery from just 50 picograms as well as significantly greater yields than with column purification methods.
Furthermore, we provide RNA Extraction kits as well as FFPE DNA Extraction Kits for use with the Bioruptor® sonication devices.
DNA purification with extraction beads
| Code | Description | Format |
|---|---|---|
| C03010010 | Auto IPure kit v2
This product must be used with the IP-Star Compact Automated System. The DNA recovery after ChIP or MeDIP might be an issue for specific downstream applications (e.g. Next generation sequencing). Diagenode’s IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP & MeDIP. |
100 rxns |
| C03010015 | IPure kit v2
Diagenode’s IPure kit is the only DNA purification kit using magnetic beads, that is specifically optimized for extracting DNA from ChIP, MeDIP and CUT&Tag. The use of the magnetic beads allows for a clear separation of DNA and increases therefore the reproducibility of your DNA purification. This simple and straightforward protocol delivers pure DNA ready for any downstream application (e.g. next generation sequencing). Comparing to phenol-chloroform extraction, the IPure technology has the advantage of being nontoxic and much easier to be carried out on multiple samples. |
100 rxns |
DNA purification on column
| Codice | Descrizione | Format |
|---|---|---|
| C03040001 | MicroChIP DiaPure columns
Diagenode’s MicroChIP DiaPure columns have been optimized for the purification and elution of very low amounts of DNA. This rapid method has been validated for epigenetic applications like low input ChIP (e.g. using the True MicroChIP kit) and CUT&Tag (e.g. using Diagenode’s pA-Tn5), but is also compatible with many other applications. The DNA can be eluted at high concentrations in volumes down to 6 μl and it is suitable for any downstream application (e.g. NGS). |
50 rxns |
RNA Extraction
| Code | Description | Format |
|---|---|---|
| C20000010 | RNA Extraction kit (Bioruptor® Plus)
The combination of the RNA extraction kit and the Bioruptor® Plus maintains the integrity of RNA and ensures maximum yield of RNA. Isolation of intact RNA is essential for many techniques used in gene expression analysis. Efficient disruption and homogenization of animal tissues are required to ensure high yield of RNA. Disruption releases RNA, while homogenization reduces sample viscosity to facilitate RNA purification. |
50 rxns |
DNA purification based on phenol-chloroform
| Code | Description | Format |
|---|---|---|
| C03030001 | DNA co-precipitant
This IP grade product is optimized to be used as carrier for DNA purification after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DNA IP). Following IP and washes, the immunoprecipitated (IP’d) material is submitted to Phenol/Chloroform/Isomanyl alcohol (PCI) extraction. Then, to the aqueous phase obtained after PCI extraction of the IP’d DNA material, our DNA co-precipitant is added with IP grade DNA precipitant (Cat. No. C03030002) and ice-cold 96% ethanol. |
100 µl |
| C03030002 | DNA precipitant
This IP grade product is used for the precipitation of DNA after chromatin immunoprecipitation (ChIP) and after methylated DNA immunoprecipitation (Methyl DNA IP). Following IP and washes, the immunoprecipitated (IP’d) material is submitted to Phenol/Chloroform/Isomanyl alcohol (PCI) extraction. Then, to the aqueous phase obtained after PCI extraction of the IP’d material, our DNA precipitant is added with IP grade DNA co-precipitant (Cat. No. C03030001) and icecold 96% ethanol. For more details about these kits, see instruction manual. |
1 ml |
| C03030020 | XL GenDNA Extraction Module
This module has been designed for the extraction of 60 µg of genomic DNA from cultured cells. The protocol and reagents allow you to perform 6 extractions of 10 µg of genomic DNA per sample, starting with 1 to 1.5 million cells. |
60 rxns |
Resin-based DNA purification
| Code | Descrizption | Format |
|---|---|---|
| C03080030 | DNA purifying slurry
The DNA purifying slurry can be used for easy and fast DNA purification and provides you with DNA suitable for qPCR analysis. If you need DNA of higher purity for next generation sequencing or other downstream application than PCR, we suggest to use the IPure kit. Diagenode’s IPure kit is the only DNA purification kit that is specifically optimized for extracting very low amounts of DNA after ChIP & MeDIP. |
10 ml |
Eluition
| Code | Descriziption | Format |
|---|---|---|
| C01010120 | Eluition Module Optimized for for the elution of immunoprecipitated DNA bound to magnetic or agarose beads. |
10 ml |
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