Chromatin Shearing Optimization Kits

The first step of ChIP – chromatin preparation – plays a key role in a succesful ChIP experiment. Each step of chromatin preparation (starting amount of material, cross-linking, cell lysis and chromatin shearing) has to be optimized, leading to a chromatin sample suitable for ChIP. A high quality chromatin sample combines two features: a suitable fragment size range and the availability of epitopes for ChIP.

Diagenode has optimized a range of solutions for successful chromatin preparation. Chromatin EasyShear Kits together with the Bioruptor ultrasonicator combine the benefits of efficient cell lysis and chromatin shearing, while keeping epitopes accessible to the antibody, critical for efficient chromatin immunoprecipitation. Each Chromatin EasyShear Kit provides optimized reagents and a thoroughly validated protocol according to your specific experimental needs. SDS concentration is adapted to each workflow taking into account target-specific requirements.

Diagenode’s Chromatin EasyShear Kits:

Highly optimized solutions for chromatin preparation validated with the Bioruptor
Recommended for the optimization of the chromatin shearing of a new cell line/new sample type prior to ChIP
Ideal for chromatin preparation prior to ChIP
Validated: Kit performance has been validated in ChIP-seq, confirming high quality of chromatin
Chromatin preparation is the first step of a ChIP assay. At Diagenode we offer complete and optimized solutions for ChIP and ChIP-seq, which include all buffers for chromatin preparation. For best results, choose your desired ChIP kit followed by the corresponding Chromatin EasyShear Kit (to optimize chromatin shearing ). The Chromatin EasyShear Kits can be used independently of Diagenode’s ChIP kits for chromatin preparation prior to any chromatin immunoprecipitation protocol. Choose an appropriate kit for your specific experimental needs:

	CELLS	Chromatin EasyShear Kit High SDS	1%
	CELLS	Chromatin EasyShear Kit Ultra Low SDS	< 0.1%
	TISSUE	Chromatin EasyShear Kit Ultra Low SDS	< 0.1%
	PLANT TISSUE	Chromatin EasyShear Kit for Plant	0.5%
	FFPE SAMPLES	Chromatin EasyShear Kit Low SDS	0.2%

	CELLS	Chromatin EasyShear Kit Low SDS	0.2%
	TISSUE
	FFPE SAMPLES

Plant-Optimized Chromatin Shearing

The first critical step of a successful ChIP experiment is the best preparation of sheared chromatin. This Chromatin EasyShear Kit is designed to be used in conjunction with the Universal Plant ChIP-seq kit and contains the right level of detergent for extraction of highest quality plant chromatin for ChIP.

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