Kits estrazione per KingFisher Flex

• Specifiche: Automated high-throughput application: Purification of up to 96 samples using the KingFisher FLEX (Thermo Fisher Scientific)
  Ideally suited for veterinary diagnostics
• Materiale di partenza: Cell-free body fluids (200 μl) – Whole blood (200 μl) – Fecal samples (50-100 mg) – Swabs from nasopharyngeal samples (e.g. Influenza testing) – Tissue samples (up to 10 mg)
• Tempi di estrazione: 60 minuti
• Resa media: in base alla tipologia e quantità materiale di partenza
• Volume eluizione: 1.8 – 2.0

• Specifiche: Use of the KingFisher FLEX automation system for running up to 96 samples in parallel – Automated elution into a 96-well plate – Includes Carrier Mix with internal DNA and RNA extraction control
• Materiale di partenza: Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl) – Cell culture supernatant, enrichment medium (up to 200 µl) – Tissues (approx. 1 – 5 mg) – Swab samples – Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Manual lysis: tissue samples only (30 – 60 minutes)
• KingFisher FLEX lysis protocol: approx. 20 minutes – KingFisher FLEX protocol: approx. 37 min.

CE-IVD

• Specifiche: Use of the KingFisher FLEX automation system for running up to 96 samples in parallel – Automated elution into a 96-well plate – Includes Carrier Mix with internal DNA and RNA extraction control
• Materiale di partenza: Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl) – Cell culture supernatant, enrichment medium (up to 200 µl) – Tissues (approx. 1 – 5 mg) – Swab samples – Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Manual lysis: tissue samples only (30 – 60 minutes)
• KingFisher FLEX lysis protocol: approx. 20 minutes – KingFisher FLEX protocol: approx. 37 minutes

• Materiale di partenza: Sangue fresco o congelato fino a 400 μL Stabilizzato in EDTA o citrato
• Tempi di estrazione: KingFisher FLEX lysis protocol: approx. 25 minutes
• KingFisher FLEX isolation protocol: approx. 37 minutes
• Resa media: in base alla tipologia e quantità materiale di partenza: Sangue intero fino a 10 μg
• Purezza media: 1.7 – 2.0

• Specifiche: For use with the KingFisher ml machines – Processing of up to 15 samples in parallel
• Materiale di partenza: Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl) – Cell culture supernatant, enrichment medium (up to 200 µl) – Tissues (approx. 1- 5 mg) – Swab samples – Stool samples (approx. 50 – 100 mg)
• Tempi di estrazione: Lysis: approx. 15 – 30 minutes, up to 60 minutes for tissue samples
• KingFisher ml protocol: approx. 30 minutes

• Specifiche: Up to 15 samples can be processed in parallel – Optimized for KingFisher ml automation systems – Includes CarrierMix with internal DNA and RNA extraction control
• Materiale di partenza:  Cell-free bodily fluids such as serum, plasma and cerebrospinal fluid (up to 200 µl) – Cell culture supernatant, enrichment medium (up to 200 µl) – Tissue samples (approx. 1- 5 mg) – Swab samples
• Tempi di estrazione: Lysis: approx. 15-30 minutes, up to 60 minutes for tissue samples.
• KingFisher ml protocol: approx. 30 minutesQuality of viral nucleic acids
• Tested with positive results in cDNA synthesis, PCR and TaqMan real-time PC

Specifiche

• Starting material: up to 200 µl ml whole blood (fresh or frozen) treated with EDTA, citrate or heparin.
• Extraction Time: approx. 56 min
• Quality: 1.7 – 2.0 **Average Yield: Depends on sample and used volume
• High Throughput Device: KingFisher Flex / KFFLX
• Extract: HMW DNA

Specifiche

• Starting material: 0.5–5 ml whole blood (fresh or frozen) treated with EDTA, citrate or heparin. Eukaryotic cells (up to 10exp7 cells)
• Extraction Time: Lysis: depending on starting material – Extraction: approx. 60 min
• Quality: 1.7 – 2.0.
• Average Yield: Depends on sample and used volume
• High Throughput Device: KingFisher Flex / KFFLX
• Extract: HMW DNA