CUT&Tag Universal Kit

Discover the Universal CUT&Tag Kit, the latest advancement in CUT&Tag technology.

Engineered to unlock high-resolution chromatin profiling for non-histone targets, including transcription factors and chromatin-associated proteins – this kit enables analysis of regulatory proteins that have traditionally been more challenging to study using standard CUT&Tag workflows.

While expanding capabilities to these targets, the kit maintains full compatibility with histone mapping, ensuring comprehensive coverage of chromatin components.

Optimized for low input and scalable to high-throughput applications, it incorporates IgG normalization and accurate library amplification to produce consistent, high-fidelity data with minimal background noise across diverse biological contexts.

Universal CUT&Tag Kit features:

• Expanded Target Range
  Robust, reproducible profiling of transcription factors and chromatin-associated proteins for comprehensive analysis of key regulatory elements.
• Comprehensive Chromatin Coverage
  Full support for histone mapping and versatile interrogation of nucleosomes and associated regulatory proteins.
• Low Input Requirement
  Reliable results from minimal material:
  – Histones: 5,000–50,000 cells
  – Chromatin-associated proteins: 10,000–100,000 cells
  – Transcription factors: 300,000 cells
• High-Throughput Ready
  Fully compatible with 96-well formats for scalable, efficient processing of large sample sets.
• Superior Data Quality
  IgG-based normalization and optimized amplification protocols minimize background and over-amplification, delivering highly reproducible data with enhanced interpretability.

For a complete CUT&Tag protocol, the following items must be purchased:

• Universal CUT&Tag Kit – includes all reagents for the CUT&Tag workflow (buffers, pA-Tn5, CoA beads, DNA purification)
• Antibody Package: anti-rabbit or anti-mouse – includes the secondary antibody, positive and negative control antibodies, and primers
• Primer indexes for tagmented libraries – for multiplexing up to 72 samples
• Primary antibody of interest – for highly specific results using Hologic Diagenode CUT&Tag-grade antibodies

Applications

• Mappatura a livello genomico delle interazioni proteina-DNA, comprese le modifiche degli istoni e le proteine regolatrici
• Genome-wide mapping of protein-DNA interactions, including histone marks and regulatory proteins
• Epigenomic studies requiring rare cell types or low cell input

Format

Sheraing Technologies	Epigenetics Antibodies

​