Takyon® Ultra Probe 4X qPCR MasterMix
Takyon® Ultra is a high-performance qPCR reagent designed for sensitive and specific DNA detection.
Our probe-based Takyon® Ultra qPCR Master Mix enables accurate, reproducible, and highly sensitive detection of DNA targets across a broad dynamic range.
The optimized formulation ensures robust performance even with complex samples, offering improved resistance to common qPCR inhibitors and compatibility with multiplex assays. The reagent supports high-throughput automation with convenient assay setup at room temperature.
Takyon® Ultra is available in various pack sizes from 200 to 10,000 – 20 µL reactions. Larger batches and custom packaging can be supplied upon request.
Advantages of Takyon® Ultra
- 4X FAST Master Mix: A concentrated format to maximize sample input, compatible with fast cycling conditions
- High Sensitivity: Detect low copy number DNA targets with excellent precision
- Improved Inhibitor Resistance: Robust amplification in the presence of common qPCR inhibitors
- Multiplex Ready: Supports simultaneous detection of multiple DNA targets
- Thermocycler Compatible: Individual ROX™ vial included for all ROX-dependent platforms
- Automation Friendly: Stable for more than 24 hours at 20 °C to simplify automated workflows
| Inhibitors | ΔCq Takyon® (standard version) | ΔCq Takyon® Ultra (new version) |
|---|---|---|
| Tannic acid (20 ng/µL) | No amplification at high inhibitor concentrations | 0.84 |
| Ethanol (5 %) | 3.39 | 1.16 |
| Hemoglobin (50 µM) | No amplification at high inhibitor concentrations | 0.23 |
| Calcium (4 mM) | 2.3 | 1.25 |
| Humic acid (20 ng/µL) | No amplification at high inhibitor concentrations | 2.45 |
Tab 1. Inhibitors were added at the indicated concentrations, and amplification was carried out using a duplex reaction targeting ACTB and 18S genes. The table displays the average ΔCq values, representing the difference in Cq between reactions with inhibitors and those without inhibitors.
Inhibitory effect
ΔCq < 2 : minor effect
2 < ΔCq < 3 : moderate effect
ΔCq > 3 : strong effect
Specifications
Products
| Codice | Descrizione |
|---|---|
| UF-UPMT-C0201 | Takyon® Ultra Probe 4X qPCR MasterMix – 200 RXN (20 µL) |
| UF-UPMT-C1001 | Takyon® Ultra Probe 4X qPCR MasterMix – 1000 RXN (20 µL) |
| UF-UPMT-C1005 | Takyon® Ultra Probe 4X qPCR MasterMix- 5×1000 RXN (20 µL) |
| UF-UPMT-C1010 | Takyon® Ultra Probe 4X qPCR MasterMix – 10×1000 RXN (20 µL) |
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