Universal Mastermix 7.5 ml

DMML-D2-D600 Universal Mastermix 7.5ml

DMML-D2-D600 Universal Mastermix 7.5ml

The Universal Mastermix (DMML-D2-D600) is a Hotstart Mastermix designed to simplify the preparation of a real-time PCR assay.

Our Universal Mastermix contains a Hotstart Taq NA polymerase, optimised reaction buffer, 4mM (final concentration) MgCl2 and nucleotides (dNTPs).

In separate tubes, passive reference (ROX), SYBR® Green, and FITC.

Avoid repeated freezing/thawing cycles.

Packaging
Kits components	Each kit contains: 2X Reaction Buffer dNTPs (including dUTP), Hotstart DNA polymerase, MgCl2 (4mM final concentration) Passive reference (500µl stock solution) SYBR® Green 1mM FlTC solution (1000µl stock solution) Passive reference stock solution for systems which require passive reference. FITC for Biorad users working with SYBR® Green.
Properties
Format	Core Kit
Quantity & Purity
Concentration	Separated components
Storage & stability
Form	In solution
Storage Conditions	For long term storage the Universal Mastermix should be stored at a temperature between -15 °C and -25°C in a constant temperature freezer. When stored under these conditions, the components are stable for a minimum of 12 months. The SYBR® Green and FITC are light sensitive and should be kept away from light as much as possible.
Activity
Application	Fluorimetric
Template type	cDNA/DNA
Volume activity	25 µL
Detection Method	SYBR Probe
Usage	Research use
Codes
Nacres Code	NA.55

Codice	Descrizione
DMML-D2-D600	Universal Mastermix 7.5ml – 600 RXN (25 µL)

Oligonucleotidi Custom

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