Megaruptor® 3

Megaruptor®3
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Megaruptor®3

Megaruptor^®3 was designed to provide the best experience with the fragmentation of DNA from 5 kb – 100 kb.

Shearing performance is independent of the source, concentration, temperature, or salt content of a DNA sample. Our user-friendly system allows for 8 samples to be processed simultaneously without additional user input. A 12 sample cassette is also available for purchase separately for increased throughput. Just set the desired parameters and the automated system takes care of the rest. Disposable hydropores do not allow problems of obstruction, prevent cross-contamination phenomena and ensure a homogeneous distribution of fragment sizes.The shearing with the Megaruptor^® 3 leads to optimal long-read sequencing using PacBio®’s, and Oxford Nanopore™ technologies’ systems.

Applications

DNA/RNA Shearing
Next Generation Sequencing

Discover the NEW Megaruptor 3 HT

The Megaruptor 3 HT offers an ultrafast protocol or increased sample processing speed to accommodate high-throughput sequencing projects. When using the ultrafast protocol, the Megaruptor 3 HT allows shearing of 96 samples in 2h into fragments of 7 to 40 kb.

The NEW Megaruptor 3 HT will give you:

High throughput: Process 96 samples in 2 h.
Flexibility: Allows the fragment size range (within 7 to 40 kb) to be chosen for hands-free operation.
Unmatched quality: Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.Unmatched quality: Provides the tight DNA fragment distribution necessary for high quality long-read sequencing.
Reliable: enables processing of highly viscous samples.
Ergonomic: Includes a user-friendly interface and has a small footprint.

Testimonials

As a PacBio Certified Service Provider it is critical that sample processing in my laboratory is precise and reproducible. For genome sequencing projects, the fragmentation of genomic DNA to precise and reproducible sizes is essential in order to optimize conditions for library preparation, sequencing, and downstream assembly. For this my laboratory relies on the Megaruptor system. The Megaruptor is the optimal system for long DNA fragment generation and tight fragment length distribution.

– Brewster Kingham, Delaware Biotechnology Institute, University of Delaware

The NGS Competence Center Tübingen (NCCT), together with four other national centers, has been established by the DFG (German Research Foundation) to support research projects with diverse needs of high-throughput sequencing technologies.

Long-read sequencing is very helpful to answer scientific questions in various topics such as microbiology or clinical research. We have noticed that the data yield of Nanopore sequencing can be notably increased by shearing the high molecular weight genomic DNA with an average size distribution of ~30kb and obtaining a read length N50 of 30kb. In this context, the Megaruptor 3 was critical to achieve long, homogenous and reproducible DNA preparation.

Megaruptor 3 is able to shear different molecular weight ranges up to 100kb; provided the input genomic DNA is of high-molecular weight. We have tested the Megaruptor 3 with genomic DNA from human blood, fibroblasts and difficult samples such as bacterial genomic DNA with high viscosity. With the Megaruptor 3 we have easily sheared up to 8 samples in parallel, saving preparation time. We have tested concentrations as low as 5 ng/µL and up to 70 ng/µL, saving sample material for optimization and meeting downstream requirements for library preparation.

Finally, handling of the Megaruptor 3 is quick, with a simple interface. Diagenode is fast in delivering consumables and these are ready-to-go. Sample preparation requires one pipetting step. You need to enter 2 parameters of your sample: volume and concentration in addition to the speed related to your desired size. It is a safe process without sample cross-contamination. It is easy to control whether the sample is going through the hydropore. The system is fast; for the concentration and speed conditions we have tested, runs were completed between half an hour and 2 hours.

– Elena Buena Atienza and Dr. Nicolas Casadei, Institute of Medical Genetics and Applied Genomics, University Clinics Tübingen

Specifications

	Megaruptor 3 regular	Megaruptor 3 HT with ultrafast protocol
Run duration	20 min – few hours	1 – 5 min
Size	5 – 100 kb	7 – 40kb
Concentration	0 – 150 ng/µl	1 – 50 ng/µl
Volume	4 – 750 µl	50 – 500 µl
Speed settings	1 – 65	30 -59
Throughput	96 samples in 1 day	96 samples in < 2h
Shearing disposables	Multiple orifice hydrodynamic pore
Hardware	State-of-the-art electronics Large memory for new features Extensive fine-tuning options Robust titanium pins