Megaruptor® 3
Megaruptor®3 è stato ideato per fornire i ricercatori di uno strumento semplice, automatico e riproducibile per la frammentazione del DNA da 5 kb – 100 kb.
La performance di Shearing è indipendente dalla sorgente, dalla temperatura e dalla concentrazione salina del campione di DNA. Il nostro software user-friendly permette il processameto di otto campioni contemporaneamente senza input addizionale dell’utilizzatore e senza cross-contaminazione. È inoltre disponibile per l’acquisto separatamente una cassetta da 12 campioni per una maggiore produttività. Basta selezionare i parametri desiderati e il sistema automatico si occupa del resto.
Gli hydropores monouso non consentono problemi di ostruzione, impediscono fenomeni di cross-contaminazione ed assicurano un omogenea distribuzione delle dimensioni dei frammenti.
I frammenti generati con Megaruptor® 3 sono analizzabili con efficacia utilizzando i sistemi PacBio® Sequel e Oxford Nanopore™.
Applicazioni
- DNA/RNA Shearing
- Next Generation Sequencing
| Scopri NEW Megaruptor 3 HT
Megaruptor 3 HT offre un protocollo ultrafast per una maggiore velocità di elaborazione dei campioni per adattarsi a progetti di sequenziamento ad alta produttività. Quando si utilizza il protocollo ultraveloce, Megaruptor 3 HT permette lo shearing di 96 campioni in 2h in frammenti da 7 a 40 kb. Il Nuovo Megaruptor 3 HT vi offrirà:
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Testimonials
As a PacBio Certified Service Provider it is critical that sample processing in my laboratory is precise and reproducible. For genome sequencing projects, the fragmentation of genomic DNA to precise and reproducible sizes is essential in order to optimize conditions for library preparation, sequencing, and downstream assembly. For this my laboratory relies on the Megaruptor system. The Megaruptor is the optimal system for long DNA fragment generation and tight fragment length distribution.
– Brewster Kingham, Delaware Biotechnology Institute, University of Delaware
The NGS Competence Center Tübingen (NCCT), together with four other national centers, has been established by the DFG (German Research Foundation) to support research projects with diverse needs of high-throughput sequencing technologies.
Long-read sequencing is very helpful to answer scientific questions in various topics such as microbiology or clinical research. We have noticed that the data yield of Nanopore sequencing can be notably increased by shearing the high molecular weight genomic DNA with an average size distribution of ~30kb and obtaining a read length N50 of 30kb. In this context, the Megaruptor 3 was critical to achieve long, homogenous and reproducible DNA preparation.
Megaruptor 3 is able to shear different molecular weight ranges up to 100kb; provided the input genomic DNA is of high-molecular weight. We have tested the Megaruptor 3 with genomic DNA from human blood, fibroblasts and difficult samples such as bacterial genomic DNA with high viscosity. With the Megaruptor 3 we have easily sheared up to 8 samples in parallel, saving preparation time. We have tested concentrations as low as 5 ng/µL and up to 70 ng/µL, saving sample material for optimization and meeting downstream requirements for library preparation.
Finally, handling of the Megaruptor 3 is quick, with a simple interface. Diagenode is fast in delivering consumables and these are ready-to-go. Sample preparation requires one pipetting step. You need to enter 2 parameters of your sample: volume and concentration in addition to the speed related to your desired size. It is a safe process without sample cross-contamination. It is easy to control whether the sample is going through the hydropore. The system is fast; for the concentration and speed conditions we have tested, runs were completed between half an hour and 2 hours.
– Elena Buena Atienza and Dr. Nicolas Casadei, Institute of Medical Genetics and Applied Genomics, University Clinics Tübingen
Specifiche
| Megaruptor 3 regular | Megaruptor 3 HT with ultrafast protocol | |
| Run duration | 20 min – few hours | 1 – 5 min |
| Size | 5 – 100 kb | 7 – 40kb |
| Concentration | 0 – 150 ng/µl | 1 – 50 ng/µl |
| Volume | 4 – 750 µl | 50 – 500 µl |
| Speed settings | 1 – 65 | 30 -59 |
| Throughput | 96 samples in 1 day | 96 samples in < 2h |
| Shearing disposables | Multiple orifice hydrodynamic pore | |
| Hardware | State-of-the-art electronics Large memory for new features Extensive fine-tuning options Robust titanium pins |
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| # | Codice Prodotto | Descrizione | Formato |
|---|---|---|---|
| 1 | B06010003 | Megaruptor® 3 | 1 unità |
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