Biosense | Staby™ SubCloning kits

Staby™ SubCloning kits

I kits Staby™ SubCloning vi permettono di trasferire il vostro gene di interesse inserito nel vettore pSTC1.3 di StabyCloning nei vettori d’espressione con nuovi vantaggi: N-term-HIS tag, N-term-Cherry tag o codoni rari tRNA (Staby™ Codon kits).

Utilizzando i siti di restrizione del vettore pSTC1.3, è facile trasferire il gene d’interesse (legato al gene antidoto ccdA) in uno dei vettori subcloning di espressione. Dal momento che la selezione Staby™ è molto efficiente, NON è necessario purificare il vostro inserto legato al gene ccdA prima del subcloning. Dopo questo processo, il vettore è pronto per l’espressione in betteri SE1.

several sub-cloning vectors

Contenuto del kit:

Vettori Staby™ SubCloning
Batteri competenti CYS21 (per cloning)
Batteri competenti SE1 (per espressione)
Medium di rigenerazione
Primers Forward e Reverse
Controllo Espressione
Manuale d’uso

Batteri competenti addizionali possono essere acquistati separatamente.

Download brochure

Descrizione	Qty	Codice
StabyTM SubCloning kit with pSSC1 vectors (Native – His – Cherry)and chemically-competent cells (cloning CYS21, expression SE1)	1 kit	GE-SSC1-12
StabyTM SubCloning kit with pSSC1 vectors (Native – His – Cherry) and electro-competent cells (cloning CYS21, expression SE1)	1 kit	GE-SSC1-10
StabyTM SubCloning kit with pSSC2 vectors (Native – His – Cherry) and chemically-competent cells (cloning CYS21, expression SE1)	1 kit	GE-SSC2-12
StabyTM SubCloning kit with pSSC2 vectors (Native – His – Cherry) and electro-competent cells (cloning CYS21, expression SE1)	1 kit	GE-SSC2-10

.Mappa Vettori

pSSC Native1 No tag. Features:T7 promoter, no tag, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning.	His Tag. Features: T7 promoter, His tag, Enterokinase cleavage site, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning.	Cherry tag. Features: T7 promoter, Cherry tag, Enterokinase cleavage site, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning.
+ rare Codon tRNA. Features: T7 promoter, no tag, additional t-RNAs, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning.	+ rare Codon tRNA, His tag. Features: T7 promoter, His tag, Enterokinase cleavage site, additional t-RNAs, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning	+ rare Codon tRNA, His tag. Features: T7 promoter, Cherry tag, Enterokinase cleavage site, additional t-RNAs, Kan resistance, lacI repressor to reduce basal expression, plasmid stabilization in CYS21 and SE1 bacteria after sub-cloning.