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Faq  CUSTOM SERVICE How much antigen do I need? What if my antigen is insoluble in biological buffers? How should I ship my antigen? How does peptide selection work for anti-peptide immunization programs? What kinds of antibody purifications are available? When should I use chicken antibodies?  OLIGO: Synthesis How are Eurogentec's oligos made and which chemistry is used? Does my oligonucleotide have a phosphate on the 5' or the 3' end? What is the longest oligonucleotide you can make? G-rich oligonucleotides, can they reliably be synthesized? How should I store my oligo and how long will it last? How do I calculate the extinction coefficient of an oligonucleotide?  REAL-TIME qPCR: EUROGENTEC MasterMix e Core Kit Why do qPCR kits contain dUTP (and UNG)? Why do the One step RT qPCR MasterMixes not contain UNG? What is the advantage of working with SYBR® Green I? What is the advantage of working with a probe system? What is the difference between a Core kit and a MasterMix? What is the difference between a MasterMix and a MasterMix Plus? Why do certain qPCR kits contain ROX passive reference? Can an "ABI" kit with a ROX passive reference be used on an IQ i-cycler? What is UNG and how does it work? In which buffer should qPCR probes be resuspended? Can Yakima Yellow™ be used with VIC settings?  CLONING & Gene Expression Why does the StabyExpress™ T7 kit contain two kinds of electrocompetent bacteria? Why does the pStaby1 plasmid contain the ampicillin resistance gene although the StabyExpress™ T7 kit allows expression without antibiotics? What is the advantage of the StabyExpress™ T7 kit if I do not find it a problem to use antibiotics (ampicillin or kanamycin) for plasmid stabilization? Why are the bacteria of the StabyExpress™ kit electrocompetent bacteria and not chemical competent bacteria? I am already using a T7 expression system from another provider. Is it necessary to change the expression conditions with the StabyExpress™ T7 kit?

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