ANTIBODIES: Custom Service

How much antigen do I need?

What if my antigen is insoluble in biological buffers?

How should I ship my antigen?

How does peptide selection work for anti-peptide immunization programs?

What kinds of antibody purifications are available?

When should I use chicken antibodies?

OLIGO: Synthesis

How are Eurogentec’s oligos made and which chemistry is used?

Does my oligonucleotide have a phosphate on the 5′ or the 3′ end?

What is the longest oligonucleotide you can make?

G-rich oligonucleotides, can they reliably be synthesized?

How should I store my oligo and how long will it last?

How do I calculate the extinction coefficient of an oligonucleotide?

REAL-TIME qPCR: Reagents

Why do qPCR kits contain dUTP (and UNG)?

Why do the One step RT qPCR MasterMixes not contain UNG?

What is the advantage of working with SYBR® Green I?

What is the advantage of working with a probe system?

What is the difference between a Core kit and a MasterMix?

What is the difference between a MasterMix and a MasterMix Plus?

Why do certain qPCR kits contain ROX passive reference?

Can an “ABI” kit with a ROX passive reference be used on an IQ i-cycler?

What is UNG and how does it work?

In which buffer should qPCR probes be resuspended?

Can Yakima Yellow™ be used with VIC settings?

PCR: Products

Does a G-Storm Cycler have a heated lid?

What is the temperature range for the G-Storm Cycler?

What is the accuracy of the temperature in a well?

What is the spread of the temperature across a block?

What cycler validation tests are available?

What do I do when the automatic lid fails to open/close?

How many programs can be stored in the memory?

What is the difference between automatic and manual hot start?

What are the temperature limits of the gradient function?

Is it possible to do Touchdown reactions?

What is the Program Wizard?

Can I export my program files?

Why does the block temperature drop to <10°C at the start of a run?

How are tubes optimally positioned in the G-Storm Cycler?

How can the G-Storm Cyclers be decontaminated?

How is a running G-Storm Cycler affected during a power failure?